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| Bortman,P.; Folgueira,M.A.A.K.; Katayama,M.L.H.; Snitcovsky,I.M.L.; Brentani,M.M.. |
| The hormone 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), the active form of vitamin D3, is an important regulator of calcium homeostasis, exerts antiproliferative effects on various cell systems and can induce differentiation in some kinds of hematopoietic cells. These effects are triggered by its receptor, vitamin D receptor (VDR), a phosphoprotein member of the nuclear receptor superfamily, which functions as a transcriptional factor. VDR binds as a heterodimer with retinoid X receptor (R X R) to hexameric repeats, characterized as vitamin D-responsive elements present in the regulatory region of target genes such as osteocalcin, osteopontin, calbindin-D28K, calbindin-D9K, p21WAF1/CIP1, TGF-ß2 and vitamin D 24-hydroxylase. Many factors such as... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calcitriol; Calcitriol receptor; Calcitriol analog; Breast tumor; Cell proliferation. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100001 |
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| Folgueira,M.A.A.K.; Federico,M.H.H.; Roela,R.A.; Maistro,S.; Katayama,M.L.H.; Brentani,M.M.. |
| A close correlation between vitamin D receptor (VDR) abundance and cell proliferation rate has been shown in NIH-3T3 fibroblasts, MCF-7 breast cancer and in HL-60 myeloblastic cells. We have now determined if this association occurs in other leukemic cell lines, U937 and K562, and if VDR content is related to c-myc expression, which is also linked to cell growth state. Upon phorbol myristate acetate (PMA) treatment, cells from the three lineages (HL-60, U937 and K562) differentiated and expressed specific surface antigens. All cell lines analyzed were growth inhibited by PMA and the doubling time was increased, mainly due to an increased fraction of cells in the G0/G1 phase, as determined by flow cytometry measurements of incorporated bromodeoxyuridine and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Receptors; Calcitriol; Cell division; Cell differentiation; Leukemia; Phorbol esters. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500011 |
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| Makdissi,F.B.A.; Machado,L.V.S.T.; Oliveira,A.G.C.; Benvenuti,T.T.; Katayama,M.L.H.; Brentani,M.M.; Osório,C.A.B.T.; Mourão Netto,M.; Lyra,E.C.; Carvalho,F.; Góes,J.C.S.; Folgueira,M.A.A.K.. |
| Epithelial intercellular cohesion, mainly mediated by E-cadherin (CDH1) expression and function, may be deregulated during cancer cell invasion of adjacent tissues and lymphatic and vascular channels. CDH1 expression is down-modulated in invasive lobular breast carcinomas but its regulation in invasive ductal carcinomas (IDC) is less clear. CDH1 expression is repressed by transcription factors such as Snail (SNAI1) and its product is degraded after Hakai ubiquitination. We compared CDH1, SNAI1 and HAKAI mRNA expression in IDC and paired adjacent normal breast tissue and evaluated its relation with node metastasis and circulating tumor cells. Matched tumor/peritumoral and blood samples were collected from 30 patients with early IDC. Epithelial cells from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; E-cadherin; Snail; Hakai. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200002 |
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| Folgueira,M.A.A.K.; Brentani,H.; Katayama,M.L.H.; Patrão,D.F.C.; Carraro,D.M.; Mourão Netto,M.; Barbosa,E.M.; Caldeira,J.R.F.; Abreu,A.P.S.; Lyra,E.C.; Kaiano,J.H.L.; Mota,L.D.; Campos,A.H.J.F.M.; Maciel,M.S.; Dellamano,M.; Caballero,O.L.S.D.; Brentani,M.M.. |
| Clinical stage (CS) is an established indicator of breast cancer outcome. In the present study, a cDNA microarray platform containing 692 genes was used to identify molecular differences between CSII and CSIII disease. Tumor samples were collected from patients with CSII or CSIII breast cancer, and normal breast tissue was collected from women without invasive cancer. Seventy-eight genes were deregulated in CSIII tumors and 22 in CSII tumors when compared to normal tissue, and 20 of them were differentially expressed in both CSII and CSIII tumors. In addition, 58 genes were specifically altered in CSIII and expression of 6 of them was tested by real time RT-PCR in another cohort of patients with CSII or CSIII breast cancer and in women without cancer.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; CDNA microarray; Clinical stage; Gene expression. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800013 |
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| Katayama,M.L.H.; Brentani,H.; Abreu,A.P.S.; Barbosa,E.M.; Oliveira,C.T.; Góes,J.C.S.; Brentani,M.M.; Folgueira,M.A.A.K.. |
| In breast cancer patients submitted to neoadjuvant chemotherapy (4 cycles of doxorubicin and cyclophosphamide, AC), expression of groups of three genes (gene trio signatures) could distinguish responsive from non-responsive tumors, as demonstrated by cDNA microarray profiling in a previous study by our group. In the current study, we determined if the expression of the same genes would retain the predictive strength, when analyzed by a more accessible technique (real-time RT-PCR). We evaluated 28 samples already analyzed by cDNA microarray, as a technical validation procedure, and 14 tumors, as an independent biological validation set. All patients received neoadjuvant chemotherapy (4 AC). Among five trio combinations previously identified, defined by nine... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Breast neoplasms; Discriminant analysis; Doxorubicin; Drug resistance; Neoadjuvant therapy; Reverse transcriptase-polymerase chain reaction. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010001200012 |
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| Roela,R.A.; Brentani,M.M.; Katayama,M.L.H.; Reis,M.; Federico,M.H.H.. |
| Cells usually lose adhesion and increase proliferation and migration during malignant transformation. Here, we studied how proliferation can affect the other two characteristics, which ultimately lead to invasion and metastasis. We determined the expression of ß1 integrins, as well as adhesion and migration towards laminin-1, fibronectin, collagens type I and type IV presented by LISP-1 colorectal cancer cells exposed to 2.5% dimethyl sulfoxide (DMSO), an agent capable of decreasing proliferation in this poorly differentiated colorectal cell line. Untreated cells (control), as shown by flow cytometry and monoclonal antibodies, expressed alpha2 (63.8 ± 11.3% positive cells), alpha3 (93.3 ± 7.0%), alpha5 (50.4 ± 12.0%) and alpha6 (34.1 ± 4.9%) integrins but... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell adhesion molecules; Cell migration; Integrin; Cell cycle; DMSO. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800016 |
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